TY - JOUR
T1 - Aberrant sphingomyelin31 p-nmr signatures in giant cell tumour of bone
AU - Quiroz-Acosta, Tayde
AU - Flores-Martinez, Yazmin Montserrat
AU - Becerra-Martínez, Elvia
AU - Pérez-Hernández, Elizabeth
AU - Pérez-Hernández, Nury
AU - Bañuelos-Hernández, Angel Ernesto
N1 - Publisher Copyright:
© 2021, Canadian Science Publishing. All rights reserved.
PY - 2021
Y1 - 2021
N2 - An understanding of the biochemistry of the giant cell tumour of bone (GCTB) provides an opportunity for the development of prognostic markers and identification of therapeutic targets. Based on metabolomic analysis, we proposed glycero-phospholipid metabolism as the altered pathway in GCTB., The objective of this study was to identify these altered metabolites. Using phosphorus-31 nuclear magnetic resonance spectroscopy (31P-NMR), sphingomyelin was determined to be the most dysregulated phospholipid in tissue samples from six patients with GCTB. Enzymes related to its biosynthesis and hydrolysis were examined using immunodetection techniques. High expression of sphingomyelin synthases 1 and 2, but low expression of neutral sphingomyelinase 2 (nSMase2) was found in GCTB tissues compared to non-neoplastic bone tissues. Sphingomyelin/ceramide biosynthesis is dysregulated in GCTB due to alterations in the expression of SMS1, SMS2, and nSMase2.
AB - An understanding of the biochemistry of the giant cell tumour of bone (GCTB) provides an opportunity for the development of prognostic markers and identification of therapeutic targets. Based on metabolomic analysis, we proposed glycero-phospholipid metabolism as the altered pathway in GCTB., The objective of this study was to identify these altered metabolites. Using phosphorus-31 nuclear magnetic resonance spectroscopy (31P-NMR), sphingomyelin was determined to be the most dysregulated phospholipid in tissue samples from six patients with GCTB. Enzymes related to its biosynthesis and hydrolysis were examined using immunodetection techniques. High expression of sphingomyelin synthases 1 and 2, but low expression of neutral sphingomyelinase 2 (nSMase2) was found in GCTB tissues compared to non-neoplastic bone tissues. Sphingomyelin/ceramide biosynthesis is dysregulated in GCTB due to alterations in the expression of SMS1, SMS2, and nSMase2.
KW - 31P-NMR
KW - Bone cancer
KW - Ceramide
KW - Giant cell tumour of bone
KW - Giant cell-rich bone tumours
KW - Glycerophospholipids
KW - Neutral sphingomyelinase 2
KW - Sphingomyelin
KW - Sphingomyelin synthase
KW - Sphingomyelinase
UR - http://www.scopus.com/inward/record.url?scp=85119984531&partnerID=8YFLogxK
U2 - 10.1139/bcb-2020-0599
DO - 10.1139/bcb-2020-0599
M3 - Artículo
C2 - 34096319
AN - SCOPUS:85119984531
SN - 0829-8211
VL - 99
SP - 717
EP - 724
JO - Biochemistry and Cell Biology
JF - Biochemistry and Cell Biology
IS - 6
ER -