A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans. © 1994, Taylor & Francis Group, LLC. All rights reserved.
Flores-Murrieta, F. J., Granados-Soto, V., & Hong, E. (1994). A simple and rapid method for determination of fluconazole in human plasma samples by high-performance liquid chromatography. Journal of Liquid Chromatography, 3803-3811. https://doi.org/10.1080/10826079408013995