A simple and rapid method for determination of fluconazole in human plasma samples by high-performance liquid chromatography

F. J. Flores-Murrieta, V. Granados-Soto, E. Hong

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Abstract

A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans. © 1994, Taylor & Francis Group, LLC. All rights reserved.
Original languageAmerican English
Pages (from-to)3803-3811
Number of pages3421
JournalJournal of Liquid Chromatography
DOIs
StatePublished - 1 Oct 1994
Externally publishedYes

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Fluconazole
High Pressure Liquid Chromatography
Ficus
Pharmaceutical Preparations
Limit of Detection
Pharmacokinetics

Cite this

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title = "A simple and rapid method for determination of fluconazole in human plasma samples by high-performance liquid chromatography",
abstract = "A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans. {\circledC} 1994, Taylor & Francis Group, LLC. All rights reserved.",
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AU - Flores-Murrieta, F. J.

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AU - Hong, E.

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N2 - A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans. © 1994, Taylor & Francis Group, LLC. All rights reserved.

AB - A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans. © 1994, Taylor & Francis Group, LLC. All rights reserved.

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