A simple and rapid method for determination of fluconazole in human plasma samples by high-performance liquid chromatography

F. J. Flores-Murrieta, V. Granados-Soto, E. Hong

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans.

Original languageEnglish
Pages (from-to)3803-3811
Number of pages9
JournalJournal of Liquid Chromatography
Volume17
Issue number17
DOIs
StatePublished - 1 Oct 1994
Externally publishedYes

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