A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents

Luis O. Soto-Rojas, Cecilia Bañuelos, Linda Garces-Ramirez, Claudia Luna-Herrera, Yazmin M. Flores-Martínez, Guadalupe Soto-Rodríguez, Bismark Gatica-García, Francisco E. López-Salas, José Ayala-Dávila, María E. Gutiérrez-Castillo, América Padilla-Viveros, Fidel de la Cruz-López, Irma A. Martínez-Davila, Daniel Martinez-Fong

    Research output: Contribution to journalArticle

    Abstract

    An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include: • No sophisticated materials or equipment are required for their application and evaluation. • They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders. • These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry.

    Original languageEnglish
    Article number100821
    JournalMethodsX
    Volume7
    DOIs
    StatePublished - 2020

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    Keywords

    • 6-OHDA
    • A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents
    • Bradykinesia
    • BSSG
    • Depressive like-behavior
    • Locomotor asymmetry
    • Parkinsonism
    • Sensorimotor alteration
    • Uncoordinated gait

    Cite this

    Soto-Rojas, L. O., Bañuelos, C., Garces-Ramirez, L., Luna-Herrera, C., Flores-Martínez, Y. M., Soto-Rodríguez, G., Gatica-García, B., López-Salas, F. E., Ayala-Dávila, J., Gutiérrez-Castillo, M. E., Padilla-Viveros, A., de la Cruz-López, F., Martínez-Davila, I. A., & Martinez-Fong, D. (2020). A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents. MethodsX, 7, [100821]. https://doi.org/10.1016/j.mex.2020.100821