TY - JOUR
T1 - A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico
AU - Anaya-Molina, Yazmin
AU - De La Cruz Hernández, Sergio Isaac
AU - Andrés-Dionicio, Atenea Estela
AU - Terán-Vega, Heidi Lizbeth
AU - Méndez-Pérez, Héctor
AU - Castro-Escarpulli, Graciela
AU - García-Lozano, Herlinda
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/12
Y1 - 2018/12
N2 - Rotaviruses continue being the most important pathogens responsible of diarrhea in young children worldwide. Seminested reverse transcription polymerase chain reaction (RT-PCR) is used to determine rotavirus genotype; however, this technique employs multistep procedures. The real-time RT-PCR is a fast and reliable tool that can be used as rotavirus genotyping tool, especially in rotavirus outbreaks. In this study, we tested a real-time RT-PCR to identify rotavirus genotype using a panel of 252 samples from patients with diarrheal disease caused by G9P[4] and G12P[8] genotypes, which were identified as emerging rotaviruses in 2 outbreaks in Chiapas, Mexico. Our results show that the real-time RT-PCR assay detected these rotaviruses, and it allowed us to identify mixed genotype infections, G/P combinations, and the viral abundance in some samples in which the seminested assay could not identify them. Therefore, the real-time RT-PCR is a molecular tool that can be great support during rotavirus outbreaks.
AB - Rotaviruses continue being the most important pathogens responsible of diarrhea in young children worldwide. Seminested reverse transcription polymerase chain reaction (RT-PCR) is used to determine rotavirus genotype; however, this technique employs multistep procedures. The real-time RT-PCR is a fast and reliable tool that can be used as rotavirus genotyping tool, especially in rotavirus outbreaks. In this study, we tested a real-time RT-PCR to identify rotavirus genotype using a panel of 252 samples from patients with diarrheal disease caused by G9P[4] and G12P[8] genotypes, which were identified as emerging rotaviruses in 2 outbreaks in Chiapas, Mexico. Our results show that the real-time RT-PCR assay detected these rotaviruses, and it allowed us to identify mixed genotype infections, G/P combinations, and the viral abundance in some samples in which the seminested assay could not identify them. Therefore, the real-time RT-PCR is a molecular tool that can be great support during rotavirus outbreaks.
KW - Genotypes
KW - Mixed rotavirus infections
KW - Real-time RT-PCR
KW - Rotavirus
UR - http://www.scopus.com/inward/record.url?scp=85050741070&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2018.06.023
DO - 10.1016/j.diagmicrobio.2018.06.023
M3 - Artículo
C2 - 30076043
SN - 0732-8893
VL - 92
SP - 288
EP - 293
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -
