A novel method for the isolation of mycobacterial DNA

Jorge A. Gonzalez-Y-Merchand, Iris Estrada-Garcia, M. Joseph Colston, Robert A. Cox

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to -70°C, then incubated at 65°C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 X 109 or more cells) including Mycobacterium neoaurum, M. fortuitum, M. phlei and M. smegmatis. Each of the species was shown to have two ribosomal RNA operons per genome, and preliminary evidence was obtained which suggests that one of these operons is homologous with one of the operons of M. smegmatis.
Original languageAmerican English
Pages (from-to)71-77
Number of pages63
JournalFEMS Microbiology Letters
DOIs
StatePublished - 1 Jan 1996

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Operon
Mycobacterium
Smegma
isolation
DNA
deoxyribonucleic acid
ribonucleic acids
Ribosomal RNA
genome
Guanidine
Ice
Chloroform
Chlorine compounds
cells
chloroform
Suspensions
ice
Genes
chlorides
Genome

Cite this

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A novel method for the isolation of mycobacterial DNA. / Gonzalez-Y-Merchand, Jorge A.; Estrada-Garcia, Iris; Colston, M. Joseph; Cox, Robert A.

In: FEMS Microbiology Letters, 01.01.1996, p. 71-77.

Research output: Contribution to journalArticle

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