A novel coconut-malt extract medium increases growth rate of morels in pure culture

Fabiola Rodríguez Evangelista, Isaac Chairez, Sigfrido Sierra, Hermilo Leal Lara, César Ramiro Martínez-González, María Eugenia Garín Aguilar, Gustavo Valencia del Toro

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Morels are gourmet wild edible mushrooms that can grow on several substrates with significant growth rate variations. Such variations have hindered the development of a standardized culture media to promote morel’s sustainable production. The aim of this study is developing a novel culture media that takes advantage of coconut water as a complementary component of culture media. Coconut water has been extensively used as a growth-promoting component for plant tissue cultures; however, its application as component of fungi cultivation medium has not been fully developed. This study confirms that coconut water can be efficiently used as culture media component for morels using a kinetic characterization. Morchella sp. kinetic growth is evaluated in different cultures: agar, malt extract agar (MEA), lactose, coconut water (15%) and combinations of them. Kinetic growth parameters (lag phase, λ and maximum specific growth rate, µmax) are estimated using primary modeling methods. Among the selected models, the best fit is achieved using Baranyi’s model. A significant increase from 15.8% to 43.4% of the µmax values was observed when culture media (agar, lactose, MEA) is supplemented with coconut water. The largest values of µmax are obtained in MEA-coconut cultures (21.13 ± 0.43–22.57 ± 0.35). Micro-sclerotia and late sclerotia are observed in all cultures containing coconut water justifying the development of a feasible and cost-effective way of culturing morels. The results demonstrate that coconut water can be used for formulation of standard media for morel cultivation leading to a cheap alternative to produce dense mycelium and promote sclerotia formation.

Original languageEnglish
Article number167
JournalAMB Express
Volume11
Issue number1
DOIs
StatePublished - Dec 2021

Keywords

  • Baranyi’s model
  • Coconut water
  • Cultivation
  • Growth rate
  • Morchella

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