A new technique to evaluate Acidithiobacillus thiooxidans growth during a bioleaching process based on DNA quantification

Andrea M. Rivas-Castillo, Marlenne Gómez-Ramírez, Isaac M. Lucas-Gómez, Yareli Carrillo-Vega, Norma G. Rojas-Avelizapa

Research output: Contribution to journalArticlepeer-review

Abstract

The potential of Acidithiobacillus (Thiobacillus) genus members, namely Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, for bioleaching purposes is known. Specifically, previous studies have shown the potential of A. thiooxidans strain DSM 26636 used in bioleaching processes to remove metals in high-metal-content matrices. All Acidithiobacillus growth-monitoring techniques available to date, including sulfate production, commonly used, present disadvantages. Thus, the current work shows a technique based on DNA quantification to evaluate the growth of A. thiooxidans DSM 26636, which is useful even in the presence of a high-metal-content residue. This proposed methodology may represent a functional complementary tool to evaluate Acidithiobacillus growth to develop biometallurgical applications.

Original languageEnglish
Article number106494
JournalJournal of Microbiological Methods
Volume198
DOIs
StatePublished - Jul 2022
Externally publishedYes

Keywords

  • Acidithiobacillus
  • Acidithiobacillus thiooxidans
  • Bioleaching
  • Culture growth
  • High-metal-content residues

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