TY - JOUR
T1 - A new technique to evaluate Acidithiobacillus thiooxidans growth during a bioleaching process based on DNA quantification
AU - Rivas-Castillo, Andrea M.
AU - Gómez-Ramírez, Marlenne
AU - Lucas-Gómez, Isaac M.
AU - Carrillo-Vega, Yareli
AU - Rojas-Avelizapa, Norma G.
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/7
Y1 - 2022/7
N2 - The potential of Acidithiobacillus (Thiobacillus) genus members, namely Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, for bioleaching purposes is known. Specifically, previous studies have shown the potential of A. thiooxidans strain DSM 26636 used in bioleaching processes to remove metals in high-metal-content matrices. All Acidithiobacillus growth-monitoring techniques available to date, including sulfate production, commonly used, present disadvantages. Thus, the current work shows a technique based on DNA quantification to evaluate the growth of A. thiooxidans DSM 26636, which is useful even in the presence of a high-metal-content residue. This proposed methodology may represent a functional complementary tool to evaluate Acidithiobacillus growth to develop biometallurgical applications.
AB - The potential of Acidithiobacillus (Thiobacillus) genus members, namely Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, for bioleaching purposes is known. Specifically, previous studies have shown the potential of A. thiooxidans strain DSM 26636 used in bioleaching processes to remove metals in high-metal-content matrices. All Acidithiobacillus growth-monitoring techniques available to date, including sulfate production, commonly used, present disadvantages. Thus, the current work shows a technique based on DNA quantification to evaluate the growth of A. thiooxidans DSM 26636, which is useful even in the presence of a high-metal-content residue. This proposed methodology may represent a functional complementary tool to evaluate Acidithiobacillus growth to develop biometallurgical applications.
KW - Acidithiobacillus
KW - Acidithiobacillus thiooxidans
KW - Bioleaching
KW - Culture growth
KW - High-metal-content residues
UR - http://www.scopus.com/inward/record.url?scp=85131356547&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2022.106494
DO - 10.1016/j.mimet.2022.106494
M3 - Artículo
C2 - 35643293
AN - SCOPUS:85131356547
SN - 0167-7012
VL - 198
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
M1 - 106494
ER -