TY - JOUR
T1 - A laboratory guide to in vitro fertilization of oceanic squids
AU - Villanueva, Roger
AU - Staaf, Danna J.
AU - Argüelles, Juan
AU - Bozzano, Anna
AU - Camarillo-Coop, Susana
AU - Nigmatullin, Chingis M.
AU - Petroni, Giuliano
AU - Quintana, Daniel
AU - Sakai, Mitsuo
AU - Sakurai, Yasunori
AU - Salinas-Zavala, César A.
AU - De Silva-Dávila, Roxana
AU - Tafur, Ricardo
AU - Yamashiro, Carmen
AU - Vidal, Erica A.G.
N1 - Funding Information:
We would like to acknowledge CIBNOR and the organizers of the 5th International Symposium on Pacific Squid for the stimulating days spent in La Paz, Mexico, which resulted in the preparation of this manuscript. RV was funded by the research project CALOCEAN ( AGL2009-11546 ) from the Ministry of Science and Innovation of Spain and RdeS by COFAA and EDI grants.
PY - 2012/4/15
Y1 - 2012/4/15
N2 - In vitro fertilization of oceanic squid is a necessary step to develop their larval culture and creates new opportunities to study and understand cephalopod development, taxonomy and ecology. The techniques described here in the form of a laboratory guide represent an attempt to refine and standardize the general methodology by indicating suitable laboratory materials, sources and preservation of gametes, and methods for fertilization and egg incubation. Twelve oceanic squid species have been fertilized in vitro to date; we outline a generalized experimental protocol and suggest that the reader consider particular species-specific modifications. Inadequate egg chorion expansion and premature hatching are identified as major challenges for in vitro fertilization. Recommendations for future research include studies on optimal gamete concentration, gamete preservation and determination of the functions of female oviducal and nidamental glands. The greatest obstacles to improving fertilization success in squids are the lack of standard methodologies and the paucity of information on both endogenous and exogenous factors controlling the fertilization process. This review is a first step toward overcoming these challenges.
AB - In vitro fertilization of oceanic squid is a necessary step to develop their larval culture and creates new opportunities to study and understand cephalopod development, taxonomy and ecology. The techniques described here in the form of a laboratory guide represent an attempt to refine and standardize the general methodology by indicating suitable laboratory materials, sources and preservation of gametes, and methods for fertilization and egg incubation. Twelve oceanic squid species have been fertilized in vitro to date; we outline a generalized experimental protocol and suggest that the reader consider particular species-specific modifications. Inadequate egg chorion expansion and premature hatching are identified as major challenges for in vitro fertilization. Recommendations for future research include studies on optimal gamete concentration, gamete preservation and determination of the functions of female oviducal and nidamental glands. The greatest obstacles to improving fertilization success in squids are the lack of standard methodologies and the paucity of information on both endogenous and exogenous factors controlling the fertilization process. This review is a first step toward overcoming these challenges.
KW - Cephalopoda
KW - Embryonic development
KW - Gametes
KW - Paralarvae
KW - Teuthida
UR - http://www.scopus.com/inward/record.url?scp=84858957127&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2012.02.025
DO - 10.1016/j.aquaculture.2012.02.025
M3 - Artículo de revisión
SN - 0044-8486
VL - 342-343
SP - 125
EP - 133
JO - Aquaculture
JF - Aquaculture
IS - 1
ER -