TY - JOUR
T1 - A glycosaminoglycan-rich fraction from sea cucumber isostichopus badionotus has potent anti-inflammatory properties in vitro and in vivo
AU - Olivera-Castillo, Leticia
AU - Grant, George
AU - Kantún-Moreno, Nuvia
AU - Barrera-Pérez, Hirian A.
AU - Montero, Jorge
AU - Olvera-Novoa, Miguel A.
AU - Carrillo-Cocom, Leydi M.
AU - Acevedo, Juan J.
AU - Puerto-Castillo, Cesar
AU - Solís, Victor May
AU - Pérez-Vega, Juan A.
AU - Gil-Zamorano, Judit
AU - Hernández-Garibay, Enrique
AU - Fernández-Herrera, María A.
AU - Pérez-Tapia, Mayra
AU - Medina-Contreras, Oscar
AU - Villanueva-Toledo, Jairo R.
AU - Rodriguez-Canul, Rossanna
AU - Dávalos, Alberto
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/6
Y1 - 2020/6
N2 - Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. Isostichopus badionotus from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that I. badionotus meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-kB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from I. badionotus of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo.
AB - Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. Isostichopus badionotus from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that I. badionotus meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-kB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from I. badionotus of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo.
KW - Ear-inflammation
KW - Glycosaminoglycans
KW - Holothuroids
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=85086147889&partnerID=8YFLogxK
U2 - 10.3390/nu12061698
DO - 10.3390/nu12061698
M3 - Artículo
C2 - 32517205
AN - SCOPUS:85086147889
SN - 2072-6643
VL - 12
SP - 1
EP - 15
JO - Nutrients
JF - Nutrients
IS - 6
M1 - 1698
ER -