TY - JOUR
T1 - A COL2A1 gene polymorphism is related with advanced stages of osteoarthritis of the knee in Mexican Mestizo population
AU - Gálvez-Rosas, Arturo
AU - González-Huerta, Celia
AU - Borgonio-Cuadra, Verónica Marusa
AU - Duarte-Salazár, Carolina
AU - Lara-Alvarado, Lorena
AU - De Los Ángeles Soria-Bastida, María
AU - Cortés-González, Socorro
AU - Ramón-Gallegos, Eva
AU - Miranda-Duarte, Antonio
N1 - Funding Information:
Acknowledgments This work was supported by a grant from the Consejo Nacional de Ciencia y Tecnología (CONACyT), México.
PY - 2010/6
Y1 - 2010/6
N2 - Primary osteoarthritis (OA) is a multifactorial disease with several genetics factors involved. The COL2A1 gene is of particular interest because it encodes for the most abundant protein in articular cartilage. The aim was to evaluate the association of COL2A1 gene polymorphism with OA of the knee in Mexican Mestizo patients. A case-control study was conducted; cases comprised patients with a radiologic scoring ≥ 2 and controls with a radiologic scoring <2. DNA was extracted from a peripheral blood sample, the polymorphic site of the COL2A1 gene was submitted to polymerase chain reaction (PCR), and the products were digested using PvuII restriction enzyme. For statistical analysis, a non-conditional logistic regression was developed. There were no associations among alleles in the overall sample, nevertheless, a significant association was found with p (Pp/pp) allele and OA of the knee grade 4 [odds ratio (OR), 95% confidence interval (CI 95%) 4.1 (1.2-14.6)] adjusted by gender, age, and body mass index (BMI). These results suggest an association of a COL2A1 gene polymorphism with advanced stages of OA of the knee in Mexican Mestizo population.
AB - Primary osteoarthritis (OA) is a multifactorial disease with several genetics factors involved. The COL2A1 gene is of particular interest because it encodes for the most abundant protein in articular cartilage. The aim was to evaluate the association of COL2A1 gene polymorphism with OA of the knee in Mexican Mestizo patients. A case-control study was conducted; cases comprised patients with a radiologic scoring ≥ 2 and controls with a radiologic scoring <2. DNA was extracted from a peripheral blood sample, the polymorphic site of the COL2A1 gene was submitted to polymerase chain reaction (PCR), and the products were digested using PvuII restriction enzyme. For statistical analysis, a non-conditional logistic regression was developed. There were no associations among alleles in the overall sample, nevertheless, a significant association was found with p (Pp/pp) allele and OA of the knee grade 4 [odds ratio (OR), 95% confidence interval (CI 95%) 4.1 (1.2-14.6)] adjusted by gender, age, and body mass index (BMI). These results suggest an association of a COL2A1 gene polymorphism with advanced stages of OA of the knee in Mexican Mestizo population.
KW - Association
KW - COL2A1 gene
KW - Osteoarthritis
KW - Polymorphism
UR - http://www.scopus.com/inward/record.url?scp=77954411692&partnerID=8YFLogxK
U2 - 10.1007/s00296-009-1091-4
DO - 10.1007/s00296-009-1091-4
M3 - Artículo
C2 - 19756630
SN - 0172-8172
VL - 30
SP - 1035
EP - 1039
JO - Rheumatology International
JF - Rheumatology International
IS - 8
ER -