A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy

L. F.E. Huerta-Nuñez, G. Gutierrez-Iglesias, A. Martinez-Cuazitl, M. M. Mata-Miranda, V. D. Alvarez-Jiménez, V. Sánchez-Monroy, Alexander Golberg, C. A. González-Díaz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Breast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.

Original languageEnglish
Article number6419
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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Dielectric Spectroscopy
Biosensing Techniques
Electric Impedance
Breast Neoplasms
Cell Line
Nanoparticles
Circulating Neoplastic Cells
Neoplasms
Magnetic Fields
Cause of Death
Spectrum Analysis
Flow Cytometry
Membrane Proteins
Monoclonal Antibodies
Neoplasm Metastasis
Recurrence
Antibodies

Cite this

Huerta-Nuñez, L. F.E. ; Gutierrez-Iglesias, G. ; Martinez-Cuazitl, A. ; Mata-Miranda, M. M. ; Alvarez-Jiménez, V. D. ; Sánchez-Monroy, V. ; Golberg, Alexander ; González-Díaz, C. A. / A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Breast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.",
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A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy. / Huerta-Nuñez, L. F.E.; Gutierrez-Iglesias, G.; Martinez-Cuazitl, A.; Mata-Miranda, M. M.; Alvarez-Jiménez, V. D.; Sánchez-Monroy, V.; Golberg, Alexander; González-Díaz, C. A.

In: Scientific Reports, Vol. 9, No. 1, 6419, 01.12.2019.

Research output: Contribution to journalArticle

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T1 - A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy

AU - Huerta-Nuñez, L. F.E.

AU - Gutierrez-Iglesias, G.

AU - Martinez-Cuazitl, A.

AU - Mata-Miranda, M. M.

AU - Alvarez-Jiménez, V. D.

AU - Sánchez-Monroy, V.

AU - Golberg, Alexander

AU - González-Díaz, C. A.

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N2 - Breast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.

AB - Breast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.

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