A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

B. P. Nobre; F. Villalobos; B. E. Barragán; A. C. Oliveira; A. P. Batista; P. A.S.S. Marques; R. L. Mendes; H. Sovová; A. F. Palavra; L. Gouveia

doi:10.1016/j.biortech.2012.11.084

A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

B. P. Nobre, F. Villalobos, B. E. Barragán, A. C. Oliveira, A. P. Batista, P. A.S.S. Marques, R. L. Mendes, H. Sovová, A. F. Palavra, L. Gouveia

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

253 Scopus citations

Abstract

The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO₂ extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33g_lipids/100g_drybiomass were found to be at 40°C, 300bar and a CO₂ flow-rate of 0.62g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO₂ doped with 20% (w/w) ethanol was used, it was possible to extract 45g_lipids/100g_drybiomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6mL/g_drybiomass.

Original language	English
Pages (from-to)	128-136
Number of pages	9
Journal	Bioresource Technology
Volume	135
DOIs	https://doi.org/10.1016/j.biortech.2012.11.084
State	Published - May 2013

Keywords

Biohydrogen
Biorefinery
Carotenoids
Nannochloropsis
Oils

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10.1016/j.biortech.2012.11.084

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Nobre, B. P., Villalobos, F., Barragán, B. E., Oliveira, A. C., Batista, A. P., Marques, P. A. S. S., Mendes, R. L., Sovová, H., Palavra, A. F., & Gouveia, L. (2013). A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass. Bioresource Technology, 135, 128-136. https://doi.org/10.1016/j.biortech.2012.11.084

@article{9b46980164514de792ef5b464a36e7d8,

title = "A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass",

abstract = "The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33glipids/100gdrybiomass were found to be at 40°C, 300bar and a CO2 flow-rate of 0.62g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45glipids/100gdrybiomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6mL/gdrybiomass.",

keywords = "Biohydrogen, Biorefinery, Carotenoids, Nannochloropsis, Oils",

author = "Nobre, {B. P.} and F. Villalobos and Barrag{\'a}n, {B. E.} and Oliveira, {A. C.} and Batista, {A. P.} and Marques, {P. A.S.S.} and Mendes, {R. L.} and H. Sovov{\'a} and Palavra, {A. F.} and L. Gouveia",

note = "Funding Information: This research was part of “Microalgae as a sustainable raw material for biofuel production (Biodiesel, Bioethanol, Bio-H 2 and Biogas)” (PTDC/AAC-AMB/100354/2008), a project sponsored by the Portuguese Foundation for Science and Technology (Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia – FCT) . Beatriz P. Nobre thanks FCT for the research grant (SFRH/BPD/42004/2007). F. Villalobos thank IPN (Project SIP 20110775) and CONACyT for a graduate scholarship. The authors would also like to thank Dr. Stephanie Seddon-Brown for the English proofreading and the reviewers for their valuable comments that have improved the manuscript quality. ",

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doi = "10.1016/j.biortech.2012.11.084",

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volume = "135",

pages = "128--136",

journal = "Bioresource Technology",

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Nobre, BP, Villalobos, F, Barragán, BE, Oliveira, AC, Batista, AP, Marques, PASS, Mendes, RL, Sovová, H, Palavra, AF & Gouveia, L 2013, 'A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass', Bioresource Technology, vol. 135, pp. 128-136. https://doi.org/10.1016/j.biortech.2012.11.084

TY - JOUR

T1 - A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

AU - Nobre, B. P.

AU - Villalobos, F.

AU - Barragán, B. E.

AU - Oliveira, A. C.

AU - Batista, A. P.

AU - Marques, P. A.S.S.

AU - Mendes, R. L.

AU - Sovová, H.

AU - Palavra, A. F.

AU - Gouveia, L.

N1 - Funding Information: This research was part of “Microalgae as a sustainable raw material for biofuel production (Biodiesel, Bioethanol, Bio-H 2 and Biogas)” (PTDC/AAC-AMB/100354/2008), a project sponsored by the Portuguese Foundation for Science and Technology (Fundação para a Ciência e a Tecnologia – FCT) . Beatriz P. Nobre thanks FCT for the research grant (SFRH/BPD/42004/2007). F. Villalobos thank IPN (Project SIP 20110775) and CONACyT for a graduate scholarship. The authors would also like to thank Dr. Stephanie Seddon-Brown for the English proofreading and the reviewers for their valuable comments that have improved the manuscript quality.

PY - 2013/5

Y1 - 2013/5

N2 - The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33glipids/100gdrybiomass were found to be at 40°C, 300bar and a CO2 flow-rate of 0.62g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45glipids/100gdrybiomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6mL/gdrybiomass.

AB - The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33glipids/100gdrybiomass were found to be at 40°C, 300bar and a CO2 flow-rate of 0.62g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45glipids/100gdrybiomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6mL/gdrybiomass.

KW - Biohydrogen

KW - Biorefinery

KW - Carotenoids

KW - Nannochloropsis

KW - Oils

UR - http://www.scopus.com/inward/record.url?scp=84876502298&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2012.11.084

DO - 10.1016/j.biortech.2012.11.084

M3 - Artículo

C2 - 23265815

SN - 0960-8524

VL - 135

SP - 128

EP - 136

JO - Bioresource Technology

JF - Bioresource Technology

ER -

A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

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Keywords

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