16S RRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting

Yendi Navarro-Noya, César Hernández-Rodríguez, Juan C. Zenteno, Beatriz Buentello-Volante, Mario E. Cancino-Díaz, Janet Jan-Roblero, Juan C. Cancino-Díaz

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalBrazilian Journal of Microbiology
Volume43
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • DGGE
  • Endophthalmitis
  • PCR
  • Staphylococcus Epidermidis

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