16S RRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting

Yendi Navarro-Noya, César Hernández-Rodríguez, Juan C. Zenteno, Beatriz Buentello-Volante, Mario E. Cancino-Díaz, Janet Jan-Roblero, Juan C. Cancino-Díaz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Original languageAmerican English
Pages (from-to)283-287
Number of pages5
JournalBrazilian Journal of Microbiology
DOIs
StatePublished - 1 Jan 2012

Fingerprint

Denaturing Gradient Gel Electrophoresis
Endophthalmitis
Microbiological Techniques
Bacteria
Culture Techniques
Polymerase Chain Reaction
Gene Library
rRNA Genes
Genes

Cite this

@article{cfec2c0ff94f49998382379084ea35f0,
title = "16S RRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting",
abstract = "Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.",
author = "Yendi Navarro-Noya and C{\'e}sar Hern{\'a}ndez-Rodr{\'i}guez and Zenteno, {Juan C.} and Beatriz Buentello-Volante and Cancino-D{\'i}az, {Mario E.} and Janet Jan-Roblero and Cancino-D{\'i}az, {Juan C.}",
year = "2012",
month = "1",
day = "1",
doi = "10.1590/S1517-83822012000100033",
language = "American English",
pages = "283--287",
journal = "Brazilian Journal of Microbiology",
issn = "1517-8382",
publisher = "Elsevier Editora Ltda",

}

TY - JOUR

T1 - 16S RRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting

AU - Navarro-Noya, Yendi

AU - Hernández-Rodríguez, César

AU - Zenteno, Juan C.

AU - Buentello-Volante, Beatriz

AU - Cancino-Díaz, Mario E.

AU - Jan-Roblero, Janet

AU - Cancino-Díaz, Juan C.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

AB - Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

U2 - 10.1590/S1517-83822012000100033

DO - 10.1590/S1517-83822012000100033

M3 - Article

SP - 283

EP - 287

JO - Brazilian Journal of Microbiology

JF - Brazilian Journal of Microbiology

SN - 1517-8382

ER -