α-Amylase production by free and immobilized Bacillus subtilis

Enrique Duran-Paramo, Oscar Garcia-Kirchner, Jean François Hervagault, Daniel Thomas, Jean Noël Barbotin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The effect of glucose on the α-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific α-amylase production in an immobilized-cell batch system, whereas a free-cell batch system presented an inversely proportional relationship with the initial glucose concentration. This might be owing to the α-amylase repression by the glucose present in the culture medium. Three hundred eighty-five percent of the specific α- amylase production with the free-cell system was produced by the immobilized- cell batch culture.

Original languageEnglish
Pages (from-to)479-485
Number of pages7
JournalApplied Biochemistry and Biotechnology
Volume84-86
DOIs
StatePublished - 2000

Keywords

  • Bacillus subtilis
  • Catabolite repression
  • Cells immobilization
  • α-Amylase production

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